Estimation Of Perichytic Photosynthesis Efficiency Using The Fluorescence Monitoring System : Response of FMS Parameters on Light Intensity, Temperature and Chlorophyll Concentrations.

The development of methods in measuring the photosynthesis process is now increasingly widespread, especially to get a more efficient method and fast. Photosynthetic efficiency of micro-algae periphytic has been estimated under the influence of light intensity and temperature by using the fluorescence monitoring system. The measurement on a colonized substrate by the saturation pulse method has been conducted using a FMS1 (Fluorescence Monitoring Systems, Hansatech). Measurement of the fluorescence parameters was conducted every week on algal periphyton which was cultivated on the artificial substrate during for 5 weeks under light and temperature conditions. The result show that fluorescence maximal value (Fm) increase linearly with chlorophyll a concentrations. For 20C (experiments where the biomass reached higher values), up to 100 mg chlorophyll a.m-2 the change in Fm is approximately linear. After, the response of Fm is hyperbolic, suggesting a saturation law. Our results clearly show that the FMS may be satisfactorily used to measure in vivo chlorophyll a fluorescence of periphyton in laboratory conditions. The instrument is simple to use, with convenient software control, especially when used in PC mode. Limnotek, Perairan Darat Tropis Di Indonesia, Vol. 17, no.1. P. 112-117 IBSN : 0854-8390